See also Genga.
After Director Checks, the final Genga is created, this stage is called nigen (二原) which is short hand for Dainigenga (第二原画) or 2nd Key. This stage incorporates all the Director Checks to create final look of anime character. Nigen is drawn without the layout paper frame.

Sample genga from Iron Saga
Nigen cut assignment folders will contain the following: